The Anti-Inflammatory Effect of Pegmatite by in Vivo and in Vitro Study.

Lee, Min Hyuk; Kim, Seok Kwun; Kwon, Yong Seok; Lee, Jang Ho; Lee, Keun Cheol

Original Article

Journal of the Korean Society of Plastic and Reconstructive Surgeons 2010;37(1):12-21.
Published online January 1, 2010.

The Anti-Inflammatory Effect of Pegmatite by in Vivo and in Vitro Study.

Min Hyuk Lee, Seok Kwun Kim, Yong Seok Kwon, Jang Ho Lee, Keun Cheol Lee

Department of Plastic & Reconstructive Surgery, College of Medicine, Dong-A University, Busan, Korea. pokdungi@dau.ac.kr

Abstract

PURPOSE
This work aimed to elucidate the anti-inflammatory effect of pegmatite in vitro and in vivo. METHODS: Author evaluated the suppressive effects of pegmatite on lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production, TNF-alpha and IL-6 release in the RAW 264.7 murine macrophages.
RESULTS
Treatment of RAW 264.7 cells with pegmatite significantly reduced LPS-stimulated NO production and inflammatory cytokine such as TNF-alpha and IL-6 secretion in a concentration-dependent manner. Also pegmatite showed topical anti-inflammatory activity in the arachidonic acid (AA)-induced ear edema and acetic acid-induced increase in capillary permeability assessment in mice. It was also found that pegmatite (10 mg per ear in DW) inhibited arachidonic acid induced edema at 24 h more profoundly than 1 h by topical application. Furthermore, the vascular permeability increase induced by acetic acid was significantly reduced in mice that received pegmatite in 50 mg per mouse.
CONCLUSION
Therefore the results of the present study suggest that pegmatite is a potent inhibitor of the LPSinduced NO and inflammatory cytokine in RAW 264.7 macrophages and showed anti-inflammatory activities in vivo animal model.

Keywords: Pegmatite; Anti-inflammatory activity; Ear edema; Vascular permeability; NO; LOX